Scientists at the Philippine Rice Research Institute (PhilRice)
have adapted a simplified method to detect viruses in plant and insect vectors
using the loop-mediated isothermal amplification (LAMP) technique.
LAMP assay has been proven
useful in detecting viruses in animals and humans, said Dr. Emmanuel Tiongco.
His team, composed of Dr. Xuan Hoai Truong, Ms. Ma. Johna Duque, and Dr.
Rubigilda Paraguison-Alili (Central Luzon State University), extended the application
of this method to rice viruses in the Philippines.
The method enables detection
of the rice tungro bacilliform virus (RTBV) in plants even before common
symptoms become noticeable.
“Through this application,
major rice virus diseases and insect vectors can be diagnosed in a fast,
efficient, and accurate method,” Dr. Tiongco said.
Using the method, RTBV can be
detected 1 day after infection. The widely used enzyme-linked immunosorbent
assay (ELISA) detects RTBV 3 days after infection.
Additionally, Dr. Tiongco
said that LAMP assay detects the rice ragged stunt virus (RRSV) and the rice
dwarf virus (RDV) not only in rice plants but also in their respective brown
planthopper and green leafhopper vectors. This way, the impending virus spread
is known even before rice crop establishment.
Existing virus detection
tools, Dr. Tiongco commented, have difficulties in detecting viruses that are
of low titer (strength of a substance’s solution) aside from being arduous and
time consuming.
Easy to follow procedures,
cheaper equipment required, high level of specificity, and simple result
determination by merely looking at the change in color of the test solution are
among the advantages of the newly developed method.
Dr. Tiongco and his team have
conducted training programs for agricultural technicians, rice researchers, and
extension workers about this new method at the PhilRice Central Experiment
Station in Nueva Ecija and its stations in Isabela, Negros, and Midsayap.
This early virus detection tool,
said Dr. Tiongco, is useful for pest control officers. The reliable and prompt
results it provides can lead to proactive solutions, and hence, prevent viral
disease epidemic.
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